Characterization of peripheral cytokine-secreting cells responses in HIV/TB co-infection

Background Currently the responses of peripheral cytokine-secreting cells in natural course human immunodeficiency virus (HIV) and tuberculosis (TB) co-infection haven’t been fully elucidated. Methods The function proinflammatory, regulatory cytotoxic were investigated by direct intracellular cytokine staining (ICS) flow cytometry, additionally, absolute numbers different measured among patients with HIV/TB (HT group), compared them healthy controls (HC TB (TB group) HIV infection (HIV group). After one week’s anti-TB treatment, changes percentages further evaluated HT groups. Results Totally 26 individuals HC group, 51 group 29 enrolled. HT. exhibited significantly lower IFN-γ + CD4 , CD8 TNF-α IL17A T CD14 monocytes than Compared secreting perforin (p=0.010; p=0.043) group. (p=0.013; p=0.001; p<0.001) decreased, percentage IL-17A (p=0.015) was increased Both TGF-β (p<0.001; p=0.001), granzyme A (all p<0.001), higher an (p=0.003) found (p=0.029) Conclusion Significantly functional profiles regulatory, observed to alone, even though those co-infection. TNF-α-secreting may be a more sensitive marker for early evaluation treatment efficacy